{"id":731,"date":"2016-02-19T21:24:21","date_gmt":"2016-02-19T20:24:21","guid":{"rendered":"http:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/?page_id=731"},"modified":"2016-11-30T19:58:16","modified_gmt":"2016-11-30T18:58:16","slug":"test-elisa-2","status":"publish","type":"page","link":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/test-elisa-2\/","title":{"rendered":"Test Elisa 2"},"content":{"rendered":"<p>On dispose<\/p>\n<ul>\n<li>d&rsquo;une barette de puits contenant un antig\u00e8ne fix\u00e9 sur le fond,<\/li>\n<li>d&rsquo;une solution \u00e0 tester (contenant ou non des anticorps sp\u00e9cifiques de l&rsquo;antig\u00e8ne pr\u00e9sent dans les puits)<\/li>\n<li>d&rsquo;une solution d\u2019anticorps \u00ab\u00a0traceurs\u00a0\u00bb, sp\u00e9cifiques des anticorps pr\u00e9c\u00e9dents et associ\u00e9s \u00e0 une enzyme, l\u2019ac\u00e9tylcholinest\u00e9rase.<\/li>\n<li>d&rsquo;une solution d\u2019Ellman (substrat de l&rsquo;ac\u00e9tylcholinest\u00e9rase). Elle se colore sous l&rsquo;action de l&rsquo;enzyme.<\/li>\n<\/ul>\n<p><a href=\"http:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-content\/uploads\/sites\/4\/2016\/02\/Test-Elisa-2.jpg\" rel=\"attachment wp-att-732\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-732 size-full\" src=\"http:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-content\/uploads\/sites\/4\/2016\/02\/Test-Elisa-2.jpg\" alt=\"Test Elisa 2\" width=\"738\" height=\"155\" srcset=\"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-content\/uploads\/sites\/4\/2016\/02\/Test-Elisa-2.jpg 738w, https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-content\/uploads\/sites\/4\/2016\/02\/Test-Elisa-2-300x63.jpg 300w\" sizes=\"auto, (max-width: 738px) 100vw, 738px\" \/><\/a><\/p>\n<p>1. Placer dans chaque puits deux gouttes d\u2019une des solutions \u00e0 tester.<br \/>\n2. Ajouter dans chacun des puits deux gouttes de la solution d\u2019anticorps traceurs.<br \/>\n3. Laisser incuber 15 min \u00e0 temp\u00e9rature ambiante.<br \/>\n4. Proc\u00e9der au lavage :<br \/>\n&#8211; vider le contenu de la barrette en la retournant \u00e9nergiquement ;<br \/>\n&#8211; absorber le liquide restant en tamponnant la barrette avec du papier filtre ;<br \/>\n&#8211; rincer \u00e0 l&rsquo;aide\u00a0 d&rsquo;une pissette d\u2019eau distill\u00e9e ;<br \/>\n&#8211; \u00e9liminer l\u2019eau de lavage en secouant \u00e9nergiquement la barrette.<br \/>\n5. R\u00e9p\u00e9ter ce lavage deux fois.<br \/>\n6. Ajouter dans chaque puits 4 gouttes (200\u00b5L) de la solution d\u2019Ellman.<br \/>\n7. Laisser agir 5 minutes.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>On dispose d&rsquo;une barette de puits contenant un antig\u00e8ne fix\u00e9 sur le fond, d&rsquo;une solution \u00e0 tester (contenant ou non des anticorps sp\u00e9cifiques de l&rsquo;antig\u00e8ne pr\u00e9sent dans les puits) d&rsquo;une solution d\u2019anticorps \u00ab\u00a0traceurs\u00a0\u00bb, sp\u00e9cifiques des anticorps pr\u00e9c\u00e9dents et associ\u00e9s \u00e0 une enzyme, l\u2019ac\u00e9tylcholinest\u00e9rase. d&rsquo;une solution d\u2019Ellman (substrat de l&rsquo;ac\u00e9tylcholinest\u00e9rase). Elle se colore sous l&rsquo;action de &hellip; <\/p>\n<p><a class=\"more-link btn\" href=\"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/test-elisa-2\/\">Lire la suite<\/a><\/p>\n","protected":false},"author":8,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-twocolumnsright.php","meta":{"footnotes":""},"class_list":["post-731","page","type-page","status-publish","hentry","nodate","item-wrap"],"_links":{"self":[{"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-json\/wp\/v2\/pages\/731","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-json\/wp\/v2\/comments?post=731"}],"version-history":[{"count":1,"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-json\/wp\/v2\/pages\/731\/revisions"}],"predecessor-version":[{"id":733,"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-json\/wp\/v2\/pages\/731\/revisions\/733"}],"wp:attachment":[{"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/svt\/wp-json\/wp\/v2\/media?parent=731"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}