{"id":2090,"date":"2020-03-03T23:42:37","date_gmt":"2020-03-03T22:42:37","guid":{"rendered":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/?p=2090"},"modified":"2024-03-11T16:56:46","modified_gmt":"2024-03-11T15:56:46","slug":"td-de-biologie-moleculaire-recherche-dune-mutation-responsable-de-la-drepanocytose","status":"publish","type":"post","link":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/2020\/03\/03\/td-de-biologie-moleculaire-recherche-dune-mutation-responsable-de-la-drepanocytose\/","title":{"rendered":"TD de Biologie mol\u00e9culaire : recherche d&#8217;une mutation responsable de la dr\u00e9panocytose"},"content":{"rendered":"<p><span style=\"font-family: trebuchet ms, geneva, sans-serif\">Cette activit\u00e9 est une activit\u00e9 Moodle de type <a href=\"https:\/\/docs.moodle.org\/3x\/fr\/Le%C3%A7on\" target=\"_blank\" rel=\"noopener noreferrer\">&#8216;le\u00e7on<\/a>&#8216;.<br \/>\nElle permet de comprendre comment d\u00e9tecter une mutation responsable de la dr\u00e9panocytose sur le g\u00e8ne de l&#8217;h\u00e9moglobine en utilisant la PCR, les enzymes de restriction et l&#8217;\u00e9lectrophor\u00e8se sur gel d&#8217;agarose<\/span><span style=\"font-family: trebuchet ms, geneva, sans-serif\">.<\/span><\/p>\n<p><span style=\"font-family: trebuchet ms, geneva, sans-serif\">Pour avoir une id\u00e9e plus pr\u00e9cise du contenu avant installation, vous pouvez t\u00e9l\u00e9charger le <a href=\"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-content\/uploads\/sites\/66\/2020\/03\/correction_TD_drepanocytose_MH_2020_2-2.pdf\">corrig\u00e9<\/a> (pdf).<br \/>\n<\/span><\/p>\n<p><span style=\"font-family: trebuchet ms, geneva, sans-serif\">L&#8217;activit\u00e9 est t\u00e9l\u00e9chargeable via le lien suivant :<\/span><br \/>\n<span style=\"font-family: trebuchet ms, geneva, sans-serif\">&#8211; <a href=\"http:\/\/hakilm.free.fr\/academie\/site_web\/ressources\/TD_drepanocytose_02-04-2021.mbz\">le\u00e7on Moodle \u00e0 installer<\/a><\/span><br \/>\n<span style=\"font-family: trebuchet ms, geneva, sans-serif\">&#8211; <a href=\"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-content\/uploads\/sites\/66\/2020\/03\/correction_TD_drepanocytose_MH_2020_2-2.pdf\">correction<\/a> (pdf)<\/span><br \/>\n<span style=\"font-family: trebuchet ms, geneva, sans-serif\">Pour installer cette le\u00e7on, t\u00e9l\u00e9charger le fichier ci-dessus. Le &#8220;restaurer&#8221; dans une le\u00e7on Moodle comme expliqu\u00e9 dans <a href=\"http:\/\/hakilm.free.fr\/academie\/lc\/restaurer\/res\/index.html\" target=\"_blank\" rel=\"noopener noreferrer\">ce tutoriel<\/a>.<br \/>\n<\/span><span style=\"font-family: trebuchet ms, geneva, sans-serif\">Une fois le le\u00e7on restaur\u00e9e dans un cours, vous pouvez la modifier, par exemple en y ajoutant des questions de type quiz (utilisateurs avanc\u00e9s).<\/span><\/p>\n<p><span style=\"font-family: trebuchet ms, geneva, sans-serif\">Dans cette \u00ab\u00a0le\u00e7on\u00a0\u00bb Moodle, les diff\u00e9rents outils utilis\u00e9s sont\u00a0:<\/span><\/p>\n<ul>\n<li><span style=\"font-family: trebuchet ms, geneva, sans-serif\">\u00ab\u00a0<a href=\"https:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi?PROGRAM=blastn&amp;PAGE_TYPE=BlastSearch&amp;LINK_LOC=blasthome\">Nucleotide blast\u00a0<\/a>\u00bb pour comparer les s\u00e9quences \u00e9tudi\u00e9es \u00e0 une base de donn\u00e9es,<\/span><\/li>\n<li><span style=\"font-family: trebuchet ms, geneva, sans-serif\">\u00ab\u00a0<a href=\"https:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi?PROGRAM=blastn&amp;PAGE_TYPE=BlastSearch&amp;LINK_LOC=blasthome\">Nucleotide blast\u00a0<\/a>\u00bb pour aligner 2 s\u00e9quences et calculer le pourcentage d\u2019identit\u00e9 entre 2 s\u00e9quences,<\/span><\/li>\n<li><span style=\"font-family: trebuchet ms, geneva, sans-serif\">\u00ab\u00a0<a href=\"https:\/\/web.expasy.org\/cgi-bin\/translate\/dna2aa.cgi\">translate<\/a>\u00bb d\u2019ExPASy pour r\u00e9aliser la traduction de s\u00e9quences nucl\u00e9otidiques (ou des outils de \u00ab\u00a0<a href=\"http:\/\/www.bioinformatics-made-simple.com\/2013\/12\/dna-to-rna-to-protein-transcription-and.html\">Bioinformatics made simple\u00a0<\/a>\u00bb),<\/span><\/li>\n<li><span style=\"font-family: trebuchet ms, geneva, sans-serif\">\u00ab\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/tools\/primer-blast\/\">primer-blast\u00a0<\/a>\u00bb pour trouver des amorces de PCR,<\/span><\/li>\n<li><span style=\"font-family: trebuchet ms, geneva, sans-serif\">\u00ab\u00a0<a href=\"http:\/\/www.bioinformatics.org\/sms2\/primer_map.html\">SMS Primer map\u00a0<\/a>\u00bb pour v\u00e9rifier les amorces choisies,<\/span><\/li>\n<li><span style=\"font-family: trebuchet ms, geneva, sans-serif\">\u00ab\u00a0<a href=\"http:\/\/nc2.neb.com\/NEBcutter2\/\">NebCutter<\/a>\u00bb pour rechercher des sites de restriction sur une s\u00e9quence d\u2019ADN et visualiser les cons\u00e9quences des coupures sur des simulations de gels d\u2019agarose.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-family: trebuchet ms, geneva, sans-serif\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cette activit\u00e9 est une activit\u00e9 Moodle de type &#8216;le\u00e7on&#8216;. Elle permet de comprendre comment d\u00e9tecter une mutation responsable de la<\/p>\n","protected":false},"author":657,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[35,33],"tags":[],"class_list":["post-2090","post","type-post","status-publish","format-standard","hentry","category-bbb","category-ressources-bgb"],"_links":{"self":[{"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-json\/wp\/v2\/posts\/2090","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-json\/wp\/v2\/users\/657"}],"replies":[{"embeddable":true,"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-json\/wp\/v2\/comments?post=2090"}],"version-history":[{"count":8,"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-json\/wp\/v2\/posts\/2090\/revisions"}],"predecessor-version":[{"id":3248,"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-json\/wp\/v2\/posts\/2090\/revisions\/3248"}],"wp:attachment":[{"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-json\/wp\/v2\/media?parent=2090"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-json\/wp\/v2\/categories?post=2090"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ent2d.ac-bordeaux.fr\/disciplines\/biotechnologies-sante-social\/wp-json\/wp\/v2\/tags?post=2090"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}